pGLO Observations , Data Recording & Analysis

pGLO Observations , Data Recording & Analysis

1

Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 400 Yellow yellow - pGLO LB/amp 0 No colonies No colonies + pGLO LB/amp 225 Yellow yellow + pGLO LB/amp/ara 200 Yellow Bright green

2.
	Two new traits transformed were that the + pGLO LB/amp/ara glows bright green and all of them grew.
3.	400 bacteria were in 100uL on each plate because there were 400 colonies in the pGLO LB.
4.
	The role of arabinose in the plates was the sugar and help the bacteria grow.
5.	Three current uses for GFP in research or applied science are first as a reporter gene to be easily expressed in bacteria, cell culture or plants and animals. The related protein can easily be measured. The second use is to create a biosensor to measure and detect an analyte. The third use is the application of less harmful fluorescent proteins such as GFP in fluorescence microscopy, studying living cells.
6.	Give an example of another application of genetic engineering. Another application of genetic engineering is glow-in-the-dark cats. This works by altering the cats DNA so that the fur of the animal can glow in the dark.